Complex target SELEX-based identification of DNA aptamers against Bungarus caeruleus venom for the detection of envenomation using a paper-based device

Complex target SELEX always have been an intriguing approach to the scientific community, as it offers potential discovery of novel biomarkers. We herein successfully performed on Bungarus caeruleus venom develop a panel highly affine aptamers that specifically recognizes B. (common krait) and was able discriminate from Cobra, Russell's, Saw-scaled viper's venom. The generated against crude also lead identification specific component venom, which is β-Bungarotoxin, toxin uniquely present in best performing aptamer candidates were used molecular recognition element paper-based device detect low 2 ng krait human serum background. developed aptamer-based paper can be for point-of-care detection applications due its simplicity affordability.